![]() ![]() In the presence of skin injury, T helper cells produce cytokines that regulate inflammatory response activity, re-epithelialization, scarification, and wound contraction. The significant impact of fast tissue healing is based on T memory cells as the first components that respond to tissue damage and recruit T cells 7. T cell activation is particularly critical during the early stages of wound healing and the scarification process 6. T cells are described as a key factor in regenerative therapy 5. The tissue healing process includes different immune cells: T cells, B cells, macrophages, monocytes, dendritic cells, neutrophils, and other components 4. Due to the limited flow cytometry analysis in veterinary regenerative medicine, we adapted cell isolation protocol from PRF samples used in human research 3. Also, it included an adaptation of cell flow cytometry protocol for canine PRF cell composition analysis. Our research was based on a new study object-canine patients with chronic inflammation and neoplastic process. However, recent findings are insufficient to support the clinical use of PRF in canines, particularly those with a history of chronic illness. Recent studies in canines commonly include histological structural analysis or growth factors expression in PRF using enzyme-linked immunosorbent assay 1, 2. The healing properties of the PRF have previously been evaluated using a variety of study approaches. Our research backs up this theory by demonstrating the difference in the levels of peripheral blood mononuclear cell (PBMC) subsets in canines’ PRF. Also, the microscopic appearance of the composition and distribution of PRF seems identical to what was detected in human research 1. The previous study in dogs and cats revealed that wound healing initiation components of platelet-rich fibrin (PRF), such as levels of different growth factors, are similar to humans 1. However, inflammatory and neoplastic processes do not affect the distribution of PBMC. Proportions of monocytes and macrophages were higher in PRF samples compared to the blood of healthy patients and periodontitis-affected patients. A higher proportion of PBMC cells related to wound healing (CD3+, CD3+ CD4+ CD8−, CD14+) were found in the PRF of control, periodontitis and neoplasia groups compared to the respective blood samples, which implies a positive outcome associated with clinical PRF usage in canine patients. ![]() In the present study, canine PRF cell composition was analyzed for the first-time using cell flow cytometry protocol. ![]() Individual clinical parameters of the patients and evaluation of the wound healing quality were included in the research. The canine patient population was divided into three subgroups: animals with periodontitis only, animals with neoplasia and periodontitis, and healthy controls. The study aimed to supplement currently available data on PRF cell composition in canine patients by isolating peripheral blood mononuclear cells (PBMC), namely T cells, matured B cells, monocytes and macrophages, and adapting current protocols of cell flow cytometry for PRF analysis. However, data reporting about its application in companion animals is lacking. Platelet-rich fibrin (PRF) is a hot research topic because of its regenerative effect in humans. ![]()
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